CDK12 (CRKRS) Protein(C62-35G)
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Description :Recombinant human CDK12 (658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :658-end
Genbank Number :NM_016507
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~135kDa
Gene Aliases :CDK12: CDK12; CRKRS; CRK7; CRKR; KIAA0904
Scientific Background :CDK12 forms a protein complex with cyclin K and this complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which play a role in DNA maintenance and repair. CDK12 is highly expressed in adult liver, kidney, testis, ovary, spinal cord and within specific adult brain regions with the highest expression being detected in cerebellum and caudate nucleus (1). CYCK-CDK12 and CYCK-CDK13 complexes show unique function with the CYCK-CDK12 playing a role in maintenance of genomic stability (2).
References :
1. Ko, T. K.et.al: CrkRS: a novel conserved Cdc2-related protein kinase that colocalises with SC35 speckles. J. Cell Sci. 114: 2591-2603, 2001.
2. Blazek, D.et.al: The cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes. Genes Dev. 25: 2158-2172, 2011.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Cancer, ERK/MAPK Pathway, Receptor Tyrosine Kinases, Cancer, Cell Cycle, Ser/Thr Kinases