BCKDK Protein(B15-31G)
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Description :Recombinant human BCKDK (30-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :30-end
Genbank Number :NM_005881
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –700C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~69 kDa
Gene Aliases :BCKDKD; BDK
Scientific Background :BCKDK or branched chain alpha-keto acid dehydrogenase kinase is an important regulator of the valine, leucine, and isoleucine catabolic pathways. BCKDK is found in the mitochondrion where it phosphorylates and inactivates BCKD. The branched-chain alpha-keto dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the oxidation of branched-chain amino acids and BCKDK inactivates the BCKDH complex by catalyzing serine phosphorylation of the E1-alpha subunit (1). BCKDH kinase catalyzes the serine phosphorylation of the E1- alpha subunit of BCKDH (2).
References :
1. Joshi, M. A. et.al: growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice. Biochem. J. 400: 153-162, 2006.
2. Popov, K. M. et.al: A. Branched-chain alpha-ketoacid dehydrogenase kinase: molecular cloning, expression, and sequence similarity with histidine protein kinases. J. Biol. Chem. 267: 13127-13130, 1992.
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Research Areas :Metabolic Disorder, Cancer, Neurobiology, Metabolic Disorder, Cardiovascular Disease, Apoptosis/Autophagy, Ser/Thr Kinases