ABL1 (K290R) Protein(A03-16NG)
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Description :Recombinant human ABL1 (K290R) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. ABL1 (K290R) is a kinase dead mutant.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :27-end
Genbank Number :NM_005157
Alternative Name(s) :ABL; JTK7; p150; c-ABL; v-abl; bcr/abl
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :ABL1protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of ABL protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene (1). Translocation and head-to-tail fusion of the BCR and ABL1 genes is present in many cases of chronic myelogeneous leukemia (2). The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1.
References :1. Barila, D. et al: An intramolecular SH3-domain interaction regulates c-Abl activity. Nature Genet. 18: 280-282, 1998.
2. Goldman, J M. et al : Targeting the BCR-ABL tyrosine kinase in chronic myeloid leukemia. New Eng. J. Med. 344: 1084-1086, 2001.
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Calculated MW ~128 kDa.
Product Sheets (By Lot #) :