INCENP Protein(I30-31H)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant human INCENP (783-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. This product can be used for Aurora B and Aurora C activation in vitro.
Species :Human
Tag :HIS tag
Expression System:Sf9 insect cells using baculovirus
Sequence :783-end
Genbank Number :NM_001040694
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~19 kDa
Gene Aliases :FLJ31633, MGC111393
Scientific Background :INCENP or inner centromere protein antigens 135/155kDa is a constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins. The overexpression of a truncation mutant of chicken INCENP and a chimeric CENPB:INCENP protein in mammalian cell culture disrupted both prometaphase chromosome alignment and completion of cytokinesis (1). INCENP forms a complex with the evolutionarily conserved family of Aurora B kinases which helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis (2).
References :
1. Mackay, A. M. et.al: A dominant mutant of inner centromere protein (INCENP), a chromosomal protein, disrupts prometaphase congression and cytokinesis. J. Cell Biol. 140: 991-1002, 1998.
2. Pereira, G. et.al: Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Science 302: 2120-2124, 2003.
Product Sheets (By Lot #) :
Research Areas :Cell Cycle, Invasion/Metastasis, Cell Cycle, Invasion/Metastasis