QIK, Active(S15-10G)
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Description :Recombinant full-length human QIK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_015191
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.activity.gif)
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~150 kDa
Gene Aliases :SNF1LK2, SIK2, KIAA0781, LOH11CR1I, DKFZp434K1115
Scientific Background :QIK is a serine/threonine protein kinase that contains an N-terminal kinase domain, a central domain with ubiquitin-associate motif, and a C-terminal PKA phosphorylation site. QIK can phosphorylate IRS1 and overexpression of QIK in adipocyte elevates the phosphorylation of IRS1 (1). The QIK-mediated phosphorylation of IRS1 may modulate the efficiency of insulin signal transduction and could be responsible for insulin resistance associated with diabetes (1). Insulin disrupts TORC2 activity by induction of QIK which then stimulates the phosphorylation and cytoplasmic translocation of TORC2. Phosphorylated TORC2 is subsequently degraded by the 26S proteasome (2).
References :
1. Horike N, et al: Adipose-specific expression, phosphorylation of ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2. J. Biol. Chem. 278: 18440-18447, 2003.
2. Dentin R, et al: Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2. Nature 449: 366-369, 2007.
Product Sheets (By Lot #) :
Research Areas :Cancer, Metabolic Disorder, Ser/Thr Kinases, Cancer, Metabolic Disorder, Ser/Thr Kinases