PRKG1, Active(P78-10BG)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant full-length human PRKG1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_006258
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~100 kDa
Gene Aliases :PGK, CGKI, PRKG1B, PRKGR1B, FLJ36117, MGC71944, cGKI-BETA, cGKI-alpha, DKFZp686K042
Scientific Background :PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain (1). By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation (2). Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP.
References :
1. Orstavik, S. et al: Characterization of the human gene encoding the type I-alpha and type I-beta cGMP-dependent protein kinase (PRKG1). Genomics 42: 311-318, 1997.
2. Li, Z. et al: A stimulatory role for cGMP-dependent protein kinase in platelet activation. Cell 112: 77-86, 2003.
Product Sheets (By Lot #) :
Research Areas :Cardiovascular Disease, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases