NEK1, Active (N02-18G)
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Description :Recombinant human NEK1 (1-477) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-477
Genbank Number :NM_012224
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~85 kDa
Gene Aliases :ALS24; SRPS2; SRTD6; SRPS2A; NY-REN-55
Scientific Background :NEK1 or NIMA (never in mitosis gene a)-related kinase 1 is a serine/threonine kinase involved in cell cycle regulation and is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. NEK1 is involved early in the DNA damage response pathway (1). NEK1 cycles through the nucleus via its nuclear localization and export signals (2). NEK1 protein participates in different signaling pathways to regulate diverse cellular processes and plays an important role in the kidney where it has opened a new avenue for studying cystogenesis and identifying possible modes of therapy.
References :
1. Polci, R. et.al: NIMA-related protein kinase 1 is involved early in the ionizing radiation-induced DNA damage response. Cancer Res. 64: 8800-8803, 2004.
2. Hilton, L. K. et.al: The NIMA-related kinase NEK1 cycles through the nucleus. Biochem. Biophys. Res. Commun. 389: 52-56, 2009.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases