MEKK2, Active(M10-10G)
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Description :Recombinant full-length human MEKK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_006609
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~115 kDa
Gene Aliases :MAP3K2, MEKK2B
Scientific Background :MEKK2 (also known as mitogen-activated protein kinase (MAPK) kinase kinase 2) is a major upstream activator of the JNK/MAPK cascade (1). MEKK2 can mediate in vitro phosphorylation of MKK4 and MKK7 which are key MAPKs that activate JNK. In addition, MEKK2 immunoprecipitates activated JUN in an IL1-dependent manner. Rheumatoid arthritis and osteoarthritis synovial tissues show high levels of MEKK2 indicating that it may be an important activator of the JNK pathway in arthritis (2). Endogenous MEKK2 is localized mainly in the cytosol of unstimulated cells.
References :
1. Blank, J. L. et al: Molecular cloning of mitogen-activated protein/ERK kinase kinases (MEKK) 2 and 3: regulation of sequential phosphorylation pathways involving mitogen-activated protein kinase and c-Jun kinase. J. Biol. Chem. 271: 5361-5368, 1996.
2. Hammaker, D. R.; Boyle, D. L.; Chabaud-Riou, M.; Firestein, G. S. : Regulation of c-Jun N-terminal kinase by MEKK-2 and mitogen-activated protein kinase kinase kinases in rheumatoid arthritis. J. Immun. 172: 1612-1618, 2004.
Product Sheets (By Lot #) :
Research Areas :ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases, ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases