ITK, Active(I13-10G)
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Description :Full-length recombinant human ITK was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_005546
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~108 kDa
Gene Aliases :EMT; LYK; PSCTK2; MGC126257; MGC126258
Scientific Background :ITK is a member of the TEC family of non-receptor tyrosine kinases. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK requires prior activation of LCK, ZAP70 and PI3-kinase for efficient activation and shares major substrates with both LCK and ZAP70 (1). ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signaling events involved in cytoskeletal reorganization (2).
References :
1. August, A. et al: The Tec family of tyrosine kinases in T cells, amplifiers of T cell receptor signals. Int J Biochem Cell Biol. 2002 Oct;34(10):1184-9.
2. Finkelstein, L D. et al: Tec kinases: shaping T-cell activation through actin. Trends Cell Biol. 2004 Aug;14(8):443-51.
Product Sheets (By Lot #) :
Research Areas :Cytoplasmic Tyrosine Kinases, Inflammation, Inflammation, Cytoplasmic Tyrosine Kinases