GAK Active (G01-11G)
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Description :Recombinant human GAK (1-396) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-396
Genbank Number :NM_005255
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Applications :Kinase Assay
Molecular Weight :~68 kDa
Gene Aliases :DNAJ26; DNAJC26
Scientific Background :Cyclin-G-associated kinase (GAK) is a serine/threonine protein kinase and was originally identified as a protein that is associated with cyclin G. However, neither CDK5 nor GAK kinase activity is activated by cyclin G nor does GAK affect CDK5 kinase activity. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
References :
1. Kanaoka Y, et al. GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain. FEBS Lett. 402(1):73-80, 1997.
2. Greener T, et al. Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells. J Biol Chem. 275(2):1365-70, 2000
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Neurobiology, Ser/Thr Kinases