AAK1, Active (A01-11G)
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Description :Recombinant human AAK1 (1-465) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-465
Genbank Number :BC002695
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :~78 kDa
Gene Aliases :KIAA1048
Scientific Background :Adaptor-associated kinase 1 (AAK1) is a serine/threonine protein kinase of the NAK kinase. Along with GAK, AAK1 regulates clathrin-mediated endocytosis by phosphorylating the μ subunits of both AP-1 and AP-2 trafficking adaptors. The phosphorylation increases the affinity of AP2 for membranes and induces a conformational change enabling cargo recruitment by this adaptor. AAK1 was also shown to play a positive role in regulating the Notch pathway.
References :
1. Gupta-Rossi N, et al. The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway. J Biol Chem. 286(21):18720-30, 2011.
2. Conner SD, Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J Cell Biol. 156(5):921-9, 2002.
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Research Areas :Ser/Thr Kinases, Neurobiology, Cancer