VRK3 Protein(V03-30G)
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Description :Recombinant full-length human VRK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_016440
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~80 kDa
Gene Aliases :(None)
Scientific Background :VRK3 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases which has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. Profound changes to the active site region underlie the loss of catalytic activity of VRK3 since it cannot bind ATP because of residue substitutions in the binding pocket (1). VRK3 is widely expressed in human tissues and the protein localizes to the nucleus. VRK3 regulates several transcription factors, nuclear envelope assembly, and chromatin condensation and is also required for cell cycle progression (2).
References :
1. Scheeff, E D. et al: Structure of the pseudokinase VRK3 reveals a degraded catalytic site, a highly conserved kinase fold, and a putative regulatory binding site. Structure. 2009 Jan 14;17(1):128-38.
2. Sanz-García M, et.al: Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities. Mol Cell Proteomics. 2008 Nov;7(11):2199-214.
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Research Areas :Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases, Cancer, ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Ser/Thr Kinases