CDK8/CyclinC Protein(C38-34G)
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Description :Recombinant full-length human CDK8/CyclinC was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC069634
Genbank Number 2 :NM_005190
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~55 kDa
Gene Aliases :CDK8: K35, MGC126074, MGC126075
CyclinC: CCNC, CycC
Scientific Background :CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (1). CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for β-catenin-driven transformation and for expression of several β-catenin transcriptional targets (2).
References :
1. Akoulitchev S, et al: TFIIH is negatively regulated by cdk8-containing mediator complexes. Nature 407: 102-106, 2000.
2. Firestein R, et al: CDK8 is a colorectal cancer oncogene that regulates beta-catenin activity. Nature 455: 547-551, 2008.
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Research Areas :Ser/Thr Kinases, Cell Cycle, Cancer