TECR-PKN1(Tex1Pex10), Active (P70-19BG)
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Description :Recombinant human fusion protein TECR (1-5a.a., exons1)-PKN1 (473a.a.-end, exons10-22) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence : 1-5a.a., exons1 (TECR) - 473a.a.-end, exons10-22 (PKN1)
Genbank Number 1:NM_138501
Genbank Number 2:NM_002741
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :80 kDa
Gene Aliases :GPSN2; MRT14; SC2; TER; PRK1, DBK, PKN1, PKN, MGC46204, PAK1, PRKCL1, PKC-L1
Scientific Background :PKN1/PRK1 belongs to the protein kinase C superfamily which is activated by Rho family of small G proteins. PKN1/PRK1 is known to mediate the Rho-dependent signaling pathway and it can be activated by phospholipids and by limited proteolysis (1). PDPK1/PDK may also mediate insulin signals to the actin cytoskeleton and the proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggest its role in signal transduction related to apoptosis. PKN1/PRK signaling stimulates AR activity in the presence of adrenal androgens and in the presence of an AR antagonist (2).
References :
1. Amano, M.et.al: Identification of a putative target for rho as the serine-threonine kinase protein kinase N. Science 271: 648-651, 1996.
2. Metzger, E. et.al: A novel inducible transactivation domain in the androgen receptor: implications for PRK in prostate cancer. EMBO J. 22: 270-280, 2003.
Product Sheets (By Lot #) :
Research Areas :Cancer