PAK2 (Y443N), Active (P03-12G)
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Description :Recombinant full-length human PAK2 (Y443N) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species: Human
Tag : N terminal GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence: Full Length
Genbank Number :NM_002577
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :~95 kDa
Gene Aliases : PAK65, PAKgamma
Scientific Background : PAK2 proteins is a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK2 (Y443N) is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell (1). The CDC42 and RAC1 induce autophosphorylation of PAK2 (Y443N), which stimulates sustained phosphorylation of other substrates (2).
References :
1. Koeppel, M. A. et.al: Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2. J. Biol. Chem. 279: 53653-53664, 2004.
2. Martin, G. A. et.al: A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20. EMBO J. 14: 1970-1978, 1995.
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Research Areas :Neurobiology, Ser/Thr Kinases, Neurobiology