TFG-MET (Tex5Mex15), Active (M52-19G)
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Description :Recombinant human TFG (1-193a.a., exons1-5)-MET (1010a.a.-end, exons15-21) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-193a.a., exons1-5 (TFG) - 1010a.a.-end, exons15-21 (MET)
Genbank Number 1:NM_001195478
Genbank Number 2:NM_000245
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :102 kDa
Gene Aliases :TFG: HMSNP; SPG57; TF6
MET: HGFR, RCCP2
Scientific Background :MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kDa (alpha) and 145 kDa (beta). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue (1). Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion (2). The fusion protein of TFG-MET has been found in the spindle cell sarcoma (3).
References :
1. Giordano, S. et al: Biosynthesis of the protein encoded by the c-met proto-oncogene. Oncogene. 1989 Nov;4(11):1383-8.
2. Iyer, A. et al: Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene. Cell Growth Differ. 1990 Feb;1(2):87-95.
3. Flucke U, et al: TFG-MET fusion in an infantile spindle cell sarcoma with neural features. Genes Chromosomes Cancer. 2017 Sep;56(9):663-667.
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Research Areas :AKT/PKB Pathway, Cancer, Cardiovascular Disease, JAK/STAT Pathway, Neurobiology, Ser/Thr Kinases