ERN1 (R727A), Active (E31-12BG)
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Description :Recombinant human ERN1 (R727A) (468-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :468-end (R727A)
Genbank Number :NM_001433
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :87 kDa
Gene Aliases :hIRE1p; IRE1; IRE1A; IRE1P
Scientific Background :ERN1 or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells (1). The activation of ERN1 through oligomerization expands the mechanistic repertoire of kinase-based signaling receptors. ERNI has a critical function in extraembryonic cells that is essential for fetal viability (2).
References :
1. Niwa, M.et.al: A role for presenilin-1 in nuclear accumulation of Ire1 fragments and induction of the mammalian unfolded protein response. Cell 99: 691-702, 1999.
2. Iwawaki, T.et.al: Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability. Proc. Nat. Acad. Sci. 106: 16657-16662, 2009.
Product Sheets (By Lot #) :
Research Areas :Angiogenesis, Cellular Stress, Neurobiology, Ser/Thr Kinases