KIAA1549-BRAF (Kex15Bex9), Active(B08-19CG)
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Description :Recombinant human fusion KIAA1549 (1320-1643 exon15) -BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1320-1643 exon15(KIAA1549) - 381-end or exon9-18 (BRAF)
Genbank Number :NM_001164665
Genbank Number 2 :NM_004333
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :~118 kDa
Gene Aliases :KIAA1549: none
BRAF: BRAF1, RAFB1, B-raf, MGC126806, MGC138284
Scientific Background :KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0 Mb tandem duplication in chromosome band 7q34 (1). The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers (2).
References :
1. Dougherty, M J. et al: Activating mutations in BRAF characterize a spectrum of pediatric low-grade gliomas. Neuro Oncol. 2010 Jul;12(7):621-30.
2. Badiali, M. et al: KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults. Brain Pathol. 2012 Nov;22(6):841-7.
Product Sheets (By Lot #) :
Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, JAK/STAT Pathway, Ser/Thr Kinases, Cancer, ERK/MAPK Pathway, JAK/STAT Pathway, Apoptosis/Autophagy, Angiogenesis, Ser/Thr Kinases