PPME1 Protein(P332-30G)
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Description :Recombinant human mature form of PPME1 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :2-end
Genbank Number :BC003046
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltrasferase Assay
Molecular Weight :70 kDa
Gene Aliases :PME-1
Scientific Background :Protein phosphatase methylesterase 1 (PPME1) conducts the demethylation of the catalytic subunit of protein phosphatase 2A (PP2A) and leads to inactivation of the phosphatase (1). It is demonstrated that PPME1 binds directly to the active site of PP2A and evicts the manganese ions required for the phosphatase activity (2). PPME1 is also reported to protect ERK activity in human malignant glioma (3).
References :
1. Ogris, E., et al. A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A. J Biol Chem. 274(20):14382-91, 1999.
2. Xing, Y., et al. Structural mechanism of demethylation and inactivation of protein phosphatase 2A. Cell. 133(1):154-63, 2008.
3. Puustinen, P., et al. PME-1 protects extracellular signal-regulated kinase pathway activity from protein phosphatase 2A-mediated inactivation in human malignant glioma. Cancer Res. 69(7):2870-7, 2009.
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Research Areas :Cancer, Neurobiology