EHMT2, Active(E318-381G)
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Description :Recombinant human EHMT2 (807-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :807-end
Genbank Number :NM_006709
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :~75 kDa
Gene Aliases :G9A, NG36, NG36/G9a
Scientific Background :EHMT2 is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1) (1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation (2). EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
References :
1. Sampath, S C. et al: Methylation of a histone mimic within the histone methyltransferase G9a regulates protein complex assembly. Molec. Cell 27: 596-608, 2007.
2. Lehnertz B. et al: Activating and inhibitory functions for the histone lysine methyltransferase G9a in T helper cell differentiation and function. J Exp Med. 2010 May 10;207(5):915-22.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle