DOT1L (KMT4), Active(D344-381G)
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Description :Recombinant human DOT1L (KMT4) (1-435) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-435
Genbank Number :NM_032482
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :78 kDa
Gene Aliases :DOT1; KMT4
Scientific Background :DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones (1, 2). DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis (3).
References :
1. Feng, Q., et al. Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain. Curr Biol. 12(12):1052-8, 2002.
2.Min, J., et al. Structure of the catalytic domain of human DOT1L, a non-SET domain nucleosomal histone methyltransferase. Cell. 112(5):711-23, 2003.
3. Okada, Y., et al. hDOT1L links histone methylation to leukemo-genesis. Cell. 121(2):167-78, 2005.
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Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease