USP20, Active (U520-381G)
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Description : Recombinant human USP20 (120-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The USP20 gene accession number is NM_006676.
Species : Human
Tag : GST tag
Expression System : Sf9 insect cells using baculovirus
Sequence : 120-end
Genbank Number : NM_006676
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 135 kDa
Gene Aliases : LSFR3A, HVDU2, VDU2, KIAA1003
Scientific Background : USP20 (ubiquitin carboxyl-terminal hydrolase 20) is a cysteine protease deubiquitinating enzyme belonging to the ubiquitin-specific protease (USP) family and deubiquitinates several proteins such as thyronine deiodinase type 2 (D2), Hypoxia-inducible factor 1α (HIF1α), and β2 adrenergic receptor. It is involved in ADRB2 recycling and resensitization, regulating thyroid hormone regulation and stabilizing and enhancing HIF1A (1). USP20 was also identified to be a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex, as it is involved in the ubiquitination and degradation of HIF1α subunits (2).
References :
1. Berthouze, M. et al: The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization. EMBO J. 28: 1684-1696, 2009.
2. Zaibo, L. et al: Identification of a deubiquitinating enzyme subfamily as substrates of the von Hippel–Lindau tumor suppressor. Biochemical and Biophysical Research Communications 294, (3): 700-709, 2002.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder