USP12, Active (U512-31G)
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Description : Recombinant human full-length USP12 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : Baculovirus in Sf9 insect cells
Sequence : Full length
Genbank Number : NM_182488
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 68 kDa
Gene Aliases : Ubiquitin specific peptidase 12
Scientific Background: Usp12 belongs to the ubiquitin-specific protease family of deubiquitinases that contains the conserved catalytic cysteine/histidine domain present in other USPs. USP12 has been identified as a deubiquitinase for histones H2A/H2B, Notch, PH domain leucine-rich repeat protein phosphatase 1, and androgen receptor (1). A complex of USP12 with WDR48 negatively regulates Akt activation, thus promoting cellular apoptosis. This complex has also been shown to suppress proliferation of tumor cell (2).
References :
1. Joo, H. Y. et al. Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46. J. Biol. Chem. 286:7190–7201, 2011.
2. Gangula, N.R. WD repeat protein WDR48 in complex with deubiquitinase USP12 suppresses Akt-dependent cell survival signaling by stabilizing PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1). J Biol Chem. 288(48):34545-34554, 2013.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder