USP5, Active (U505-380H)
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Description : Recombinant full-length human USP5 was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : Full length
Genbank Number : NM_003481
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 98 kDa
Gene Aliases : ISOT, Isopeptidase T
Scientific Background : USP5 (ubiquitin carboxyl-terminal hydrolase 5) is a member of the ubiquitin-specific protease family of deubiquitinating enzymes (DUBs) and is involved in many processes including maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism at the proximal end of the chain. Increased USP5 has been associated with tumour formation and progression of pancreatic cancer due to its role in FoxM1 protein stabilization (1). USP5 is also involved in the upregulation of Notch signaling and downregulation of receptor tyrosine kinase signaling in Drosophila eye development through the deubiquitination of unanchored polyubiquitin (2).
References :
1. Li, X.Y. et al: USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein. Biochem. Biophys. Res. Commun. 492:48-54, 2017.
2. Ling, X. et al: The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development. FEBS 591(6): 875-888, 2017.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder