CRBN/DDB1/CUL4A/RBX1 Complex(U273-380H)
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Description :Recombinant full-length human CRBN, DDB1, CUL4A, and RBX1 were expressed by baculovirus in Sf9 insect cells using N-terminal His-tags for all proteins.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :CRBN: Q96SW2
Genbank Number 2 :DDB1: Q16531
Genbank Number 3 :CUL4A: Q13619
Alternative Name(s) :CRBN: MRT2; MRT2A; protein cereblon
DDB1: DDBA; UV-DDB1; WHIKERS; XAP1; XPCE; XPE; DNA damage-binding protein 1
CUL4A: cullin-4A; CUL-4A
RBX1: RING-box protein 1; E3 ubiquitin-protein ligase RBX1; Protein ZYP
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :The CRBN/DDB1/CUL4A/RBX1 complex, also known as CRL4 CRBN E3 ubiquitin ligase complex, forms an E3 ubiquitin ligase that tags target proteins for ubiquitin-mediated degradation, thereby regulating various cellular processes such as cell cycle progression, DNA repair, and protein homeostasis. CRBN is a substrate receptor that serves as a binding site for specific target proteins, allowing the complex to recognize and ubiquitinate them for degradation. DDB1 acts as an adaptor protein that connects CRBN to CUL4A within the complex. CUL4 acts as a scaffold within the complex, providing the structural backbone for the assembly of the complex and facilitates the transfer of ubiquitin molecules to the target proteins. RBX1 interacts with CUL4A and facilitates the transfer of ubiquitin moieties to the target proteins, marking them for degradation by the proteasome.
References :1. Ito T, et al. (2010). Identification of a primary target of thalidomide teratogenicity. Science, 327(5971), 1345-1350.
2. Sugasawa K, et al. (2005). A multistep damage recognition mechanism for global genomic nucleotide excision repair. Genes & Development, 19(17), 2192-2203.
Molecular Weight :Subunit Observed MW (~kDa) Calculated MW (~kDa)
CRBN 58 51
DDB1 128 131
CUL4A 88 87
RBX1 14 12
Product Sheets (By Lot #) :