VHL/ELOB/ELOC/CUL2/RBX1 complex, Active(U272-380H)
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Description :Recombinant full-length human VHL, ELOB, ELOC, CUL2, and RBX1 were expressed by baculovirus in Sf9 insect cells using N-terminal His-tags for all proteins.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :VHL: NM_000551.3
Genbank Number 2 :ELOB: NM_007108.3
Genbank Number 3 :ELOC: NM_001204857.1
Alternative Name(s) :• VHL: von Hippel-Lindau disease tumor suppressor; protein G7; pVHL; HRCA1; RCA1; VHL1
• ELOB: Elongin-B; TCEB2; SIII p18; RNA polymerase II transcription factor SIII subunit B
• ELOC: Elongin-C; TCEB1; SIII; RNA polymerase II transcription factor SIII subunit C
• CUL2: Cullin-2; MGC131970
• RBX1: RING-box protein 1; E3 ubiquitin-protein ligase RBX1; Protein ZYP
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :The VHL/ELOB/ELOC/CUL2/RBX1 complex, also known as the VBC complex, is an E3 ubiquitin ligase that plays a critical role in the regulation of the hypoxia-inducible factor (HIF) pathway. HIF is a transcription factor that is activated in response to low oxygen levels and regulates the expression of genes involved in angiogenesis, erythropoiesis, and metabolism. Under normoxic conditions, HIF is targeted for degradation by the VBC complex, which recognizes and ubiquitinates HIF for proteasomal degradation. Dysregulation of the HIF pathway is also a hallmark of many cancers and targeting the VBC complex has emerged as a promising therapeutic strategy for the treatment of cancer.
References :1. Ohh M.: Ubiquitin pathway in VHL cancer syndrome. Cancer Cell. 10(2):77-78, 2006.
2. Min, J.H. et al: Structure of an HIF-1alpha-pVHL complex: hydroxyproline recognition in signaling. Science. 296(5574):1886-1889, 2002.
Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Subunit Observed MW (~kDa) Calculated MW (~kDa) VHL 29 24 ELOB 18 13.4 ELOC 14 12.7 CUL2 94 85 RBX1 14 12.3
Product Sheets (By Lot #) :