ULK1, Active(U01-11G)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-649
Genbank Number :BC111603
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.activity.gif)
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~125 kDa
Gene Aliases :ATG1, UNC51, Unc51.1, FLJ38455
Scientific Background :ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR (1). ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (2). Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
References :
1. Ganley I G, et al: ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy. J Biol Chem. 2009 May 1;284(18):12297-305.
2. Chan E Y, et al: siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem. 2007 Aug 31;282(35):25464-74.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Ser/Thr Kinases, Cancer, Apoptosis/Autophagy, Ser/Thr Kinases