TEV Protease, Active(T525-31BH)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant TEV Protease protein (aa 1-237) from Tobacco etch virus was expressed in E. coli using a C-terminal His tag
Species :TE Virus
Tag :HIS
Expression System:E.coli
Sequence :aa 1-237
Genbank Number :M15239
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Alternative name(s) :rTEV, TEV, P1 protease, Tobacco Etch Virus nuclear-inclusion-a endopeptidase. Uniprot accession Q0GDU8
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 250mM NaCl, 1mM DTT, 10% glycerol.
Storage and Stability :Aliquot product into smaller quantities after centrifugation and store at –70oC. For best performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :TEV Protease is a highly sequence specific cystine protease originating from the Tobacco Etch Virus (1). The optimum recognition site for this enzyme is the sequence ENLYFQ(G/S) and cleavage occurs between the Gln and Gly/Ser residues (2,3). It is frequently used in affinity purification of proteins to remove affinity tags from recombinant proteins.
References :1. Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK 3rd, Kapust RB, Li M, Wlodawer A, Waugh DS. 2002. Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem. 27;277(52):50564-72.
2. Kostallas G, Löfdahl PÅ, Samuelson P. 2011. Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay. PLoS One. 6(1):e16136.
3. Carrington JC, Dougherty WG. 1988. A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc Natl Acad Sci USA. 85(10):3391-5.
Molecular Weight :Observed MW ~28 kDa. Calculated MW ~28 kDa
Product Sheets (By Lot #) :