TTK, Active(T20-10G)
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Description :Recombinant full-length human TTK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_003318
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.activity.gif)
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~130 kDa
Gene Aliases :ESK, PYT, MPS1, MPS1L1, FLJ38280
Scientific Background :TTK is a serine/threonine kinase that has been implicated in the regulation of centrosome duplication and mitotic checkpoint response. Overexpressing of dominant-negative TTK in human cell lines prevents centrosome duplication while active TTK accelerates centrosome reduplication in an osteosarcoma cell line. Disruption of TTK function leads to a combination of severe mitotic abnormalities and failure in centrosome duplication (1). TTK is required for stabilization and activation of p53 during spindle disruption. TTK phoshorylates the N-terminal domain of p53 at Thr18, and this phosphorylation disrupts the interaction with MDM2 and abrogates MDM2-mediated p53 ubiquitination (2).
References :
1.Fisk H A, et al: Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression. Proc. Nat. Acad. Sci. 100: 14875-14880, 2003.
2.Huang Y F, et al: TTK/hMps1 mediates the p53-dependent postmitotic checkpoint by phosphorylating p53 at Thr18. Mol Cell Biol. 2009 Jun;29(11):2935-44.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cytoplasmic Tyrosine Kinases, Cancer, Cell Cycle, Ser/Thr Kinases, Cytoplasmic Tyrosine Kinases