SIRT2, Active(S36-30H)
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Description :Recombinant full length human SIRT2 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species :Human
Tag :HIS tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_030593
Specific Activity :Sample SIRT Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :SIRT Assay
Molecular Weight :~42 kDa
Gene Aliases :SIR2L, SIR2L2, Sirtuin 2
Scientific Background :SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation.
References :
1. Luthi-Carter, R. Et al: SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7927-32.
2. North, B. J. et al: The human Sir2 ortholog, SIRT2, is an NAD(+)-dependent tubulin deacetylase. Molec. Cell 11: 437-444, 2003.
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