PSMD14 Protein (P501-30G)
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Description : Recombinant full-length human PSMD14 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : Sf9 insect cells using baculovirus
Sequence : Full Length
Genbank Number : NM_005805
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : Approx. 61 kDa
Gene Aliases : Proteasome 26S Subunit, Non-ATPase, POH1, 26S Proteasome Non-ATPase Regulatory Subunit
Scientific Background : PSMD14 is a deubiquitinating enzyme within the 19S proteasomal subunit. It is specific for K63-Ub chains releasing polyubiquitin chains from the polyubiquitinated protein substrates of the proteasome. PSMD14 regulates macroautophagy by controlling Golgi-to-ER retrograde transport (1). It also plays a critical role in physiological and pathological processes such as DNA damage repair, tumor formation and inflammation (2). PSMD14 is significantly upregulated in HCC and lung cancer, and indicates poor prognosis (2, 3). Downregulation of PSMD14 causes cell cycle arrest leading to senescence and apoptosis, therefore it is a potential target for small molecule inhibitors in cancer therapeutics (2).
References :
1. Bustamante, H.A. et al: The proteasomal deubiquitinating enzyme PSMD14 regulates macroautophagy by controlling Golgi-to-ER retrograde transport. Cells 2020; 9(3), 777.
2. Zhang, L. et al: Upregulation of deubiquitinase PSMD14 in lung adenocarcinoma (LUAD) and its prognostic significance. Journal of Cancer. 2020; 11(10):2962-2971.
3. Jie, L. et al: Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2. Cancer Letters. 2020; 469:22-34.
Product Sheets: (by Lot #):
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder