PDE12, Active(P100-310G)
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Description :Recombinant full-length human PDE12 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_177966
Alternative Name(s) :2'-PDE
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :PDE12 is 2', 5'-phosphodiesterase 12 protein that belongs to the CCR4/nocturin family. PDE12 cleaves the 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates. Triphosphorylated oligoadenylates, which are referred to as 2-5A, modulates in the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs) (1). Suppression of PDE12 induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.
References :1. Kubota K. et.al: "Identification of 2'-phosphodiesterase, which plays a role in the 2-5A system regulated by interferon." J. Biol. Chem. 279:37832-37841,2004.
Specific Activity :Sample Phosphodiesterase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Approx. MW 100kDa.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle