WIP1, Active (P04-20G)
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Description :Recombinant full-length human WIP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC016480
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Phosphatase Assay, Western Blot
Molecular Weight :110 kDa
Gene Aliases :PPM1D; JDVS; IDDGIP; PP2C-DELTA
Scientific Background :20mM MOPS, pH 7.5, 300mM NaCl, 10mM glutathione, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
References :
1. Fiscella M. et. al: Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner. PNAS. 1997 Jun 10; 94(12):6048-53.
2. Yoda A. et. al: Intrinsic kinase activity and SQ/TQ domain of Chk2 kinase as well as N-terminal domain of Wip1 phosphatase are required for regulation of Chk2 by Wip1. J Biol Chem. 2006 Aug 25; 281(34):24847-62.
3. Moon S. H. et. al: Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. J Biol Chem. 2010 Apr 23; 285(17):12935-47.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, Neurobiology, NfkB Pathway, p38 Pathway, Ser/Thr Kinases