OTULIN, Protein (O585-30H)
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Description : Recombinant full-length human OTULIN was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : Full length
Genbank Number : NM_138348
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 49 kDa
Gene Aliases : GUM; AIPDS; FAM105B
Scientific Background : OTULIN (OTU deubiquitinase with linear linkage specificity) belongs to the family of peptidase C65 family of ubiquitin isopeptidases via removing ubiquitin from proteins (1). OTULIN specifically targets on Met1-linked polyubiquitin chains to repress the linear ubiquitin chain assembly complex (LUBAC) mediated ubiqitination (2). OTULIN deficiency leads to a TNF-related systemic inflammation and aotuimmunity in humans and mice model, and the neutralizing antibody can rescue the phenotypes (3).
References :
1. Mevissen TE, et al. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis. Cell. 2013 Jul 3;154(1):169-84.
2. Keusekotten K, et al. OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1-linked polyubiquitin. Cell. 2013 Jun 6;153(6):1312-26.
3. Damgaard RB, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016 Aug 25;166(5):1215-1230.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder