OTUB1, Protein (O568-30H)
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Description : Recombinant full-length human OTUB1 was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : Full length
Genbank Number : NM_017670
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : 35 kDa
Gene Aliases : OTB1; OTU1; HSPC263; OTU deubiquitinase, ubiquitin aldehyde binding 1
Scientific Background : OTUB1 (OTU domain-containing ubiquitin aldehyde-binding protein 1), as a member of the ovarian tumor (OUT) superfamily of predicted cysteine proteases (1). It specifically cleaves ubiquitin from branched lysine48-linked poly-ubiquitin chains (1). OTUB1 inhibits the synthesis of the K63-linked polyubiquitin chains through binding to the UBC13 (2). Overexpression of OTUB1 is associated to poor outcome in some breast cancer patients with chemotherapy (3).
References :
1. Edelmann MJ, et al. Structural basis and specificity of human otubain 1-mediated deubiquitination. Biochem J. 2009 Mar 1;418(2):379-90.
2. Wiener R, et al. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22.
3. Karunarathna U, et al. OTUB1 inhibits the ubiquitination and degradation of FOXM1 in breast cancer and epirubicin resistance. Oncogene. 2016 Mar 17;35(11):1433-44.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder