NEDD8, Protein (N263-31H)
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Description : Recombinant human NEDD8 (1-76 a. a.) was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E. coli cells
Sequence : 2-157 a. a.
Genbank Number : NM_006156
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : 10 kDa
Gene Aliases : NEDD-8
Scientific Background : Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) encodes a ubiquitin-like protein (1). NEDD8 can be conjugated to proteins via the C-terminal Gly by the E2 enzyme Ubc12 and E3 ligase complex to affect various signaling molecules such as cullins, auxin, IRF3, MDM2, and p21(2). On the contrary, the NEDD8 modified substrates can be reversed by deneddylating enzymes such as CSN, NEDP1, UAP21, Ataxin3, PfUCH54, UCHL1 and UCHL3 (3). The inhibitor of NEDD8 suppressed the tumor growth in AML cells in xenograft assays (3).
References :
1. Kamitani, T, et al. Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. J. Biol. Chem. 272, 28557–28562 (1997).
2. Schmidt MHH, et al. Ubiquitin and NEDD8: brothers in arms. Sci STKE. 2006 Nov 21;2006(362).
3. Watson IR, et al. NEDD8 pathways in cancer, Sine Quibus Non. Cancer Cell. 2011 Feb 15;19(2):168-76.
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder