NEK4, Active(N06-10G)
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Description :Recombinant full-length human NEK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC063044
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~160 kDa
Gene Aliases :NRK2, STK2
Scientific Background :NEK4 or Never In Mitosis A-related kinase 4 is a serine/threonine protein kinase. Northern blot show NEK4 is expressed as 2 mRNAs of 4.0 kb and 6.8 kb in heart and several other tissues. NEK4 gene encodes an 841-amino acid polypeptide and the levels of NEK4 mRNA remain invariant throughout the cell cycle (1). Nek4 has been identified in a genetic screen as a mediator of response to Taxol (2). After Taxol treatment, NEK4 promoted microtubule outgrowth, whereas NEK4 deficiency impaired G(2)-M arrest and decreased formation of mitotic-like asters. In contrast, NEK4 deficiency sensitized cells to vincristine, which destabilizes microtubules.
References :
1. Levedakou, E. N., et.al: Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern. Oncogene 9: 1977-1988, 1994.
2. Doles, J. et al: Nek4 status differentially alters sensitivity to distinct microtubule poisons. Cancer Res. 2010 Feb 1;70(3):1033-41.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases