ASK1, Active(M13-11G)
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Description :Recombinant human ASK1(649-946) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :649-946
Genbank Number :NM_005923
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~60 kDa
Gene Aliases :MAP3K5; MEKK5; MAPKKK5
Scientific Background :ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
References :
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
Product Sheets (By Lot #) :
Research Areas :Cancer, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases, Cancer, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases