Lys-c (lysyl-endopeptidase), Active(L585-31H)
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Description :Recombinant Achromobacter lyticus Lys-c (lysyl-endo-peptidase) (206-473aa) was expressed in E. coli cells using a C-terminal His tag.
Species :Achromobacter Lyticus
Tag :HIS
Expression System:E.coli
Sequence :206-473aa
Genbank Number :The enzyme commission number is EC 3.4.21.50.
Alternative Name(s) :API, Protease I, Lysyl endopeptidase
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 8.0, 30% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Digestion Conditions :Catalytic pH range: 9.0 ~ 9.5 Catalytic temperature range: 30 ~ 37oC
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Scientific Background :Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Masaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis. The enzyme functions optimally between 30 – 37oC and suffers from degradation when subjected to temperatures above 50oC. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30oC. Conversely, the enzyme is inhibited by DFP, PMSF, TLCK. Lysyl-endopeptidase is involved during the production of recombinant insulin and in proteomics research.
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