Lambda Protein Phosphatase (L01-20U)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant full-length untagged Bacteriophage lambda Lambda Protein Phosphatase was expressed in E. coli.
Species :Bacteriophage Lambda
Tag :
Expression System:E.coli
Sequence :Full length
Genbank Number :P03772.1
Alternative name (s) :Lambda PP, Serine/Threonine-protein phosphatase
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 1mM DTT, 10% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Lambda Protein Phosphatase (lambda PP) is a Mn 2-dependent protein phosphatase composed of 221 amino acids and is encoded on ORF211 of bacteriophage lambda (1). Lambda PP has activity towards phosphorylated serine, threonine, tyrosine and histidine residues (2). It can be inhibited by vanadate ions (3).
References :1. Cohen, P.T.W., and Cohen, P. 1989. Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221. Biochem. J. 260, 931-934.
2. Zhuo S et al. 1993. Expression, purification, crystallization, and biochemical characterization of recombinant protein phosphatase. J. Biol. Chem. 268:17754-17761.
3. Gordon JA. 1991. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol. 201:477-82.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Specific Activity :Specific Activity The specific activity of endonuclease was determined to be 613,000 Units/ml as per the activity assay protocol.
Unit Definition:
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50mM) in 1 minute at 30°C in a total reaction volume of 50µl.
Molecular Weight :Calculated MW 25.2 kDa
Product Sheets (By Lot #) :