GSK3 beta, Active(G09-10G)
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Description :Recombinant full-length human GSK3beta was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_001146156
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~73 kDa
Scientific Background :GSK3β is a serine threonine protein kinase that was originally identified as the kinase that phosphorylate and inhibits glycogen synthase (1). GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3 beta into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies (2).
References :
1. Sutherland C. et al: Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling. Biochem J. 1993 Nov 15;296 ( Pt 1):15-9.
2. Sperber BR, Leight S, Goedert M, Lee VM. Glycogen synthase kinase-3 beta phosphorylates tau protein at multiple sites in intact cells. Neurosci Lett. 1995 Sep 8;197(2):149-53.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Invasion/Metastasis, Ser/Thr Kinases, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Invasion/Metastasis