CDC42, Active (C108-310GH)
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Description : Recombinant full-length human CDC42 was expressed in E.coli cells using an N-dual GST/His tag.
Species : Human
Tag : GST/HIS
Expression System : E.coli
Sequence : Full length
Genbank Number : NM_001791
Specific Activity : Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability, and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Enzyme Assay, Western Blot
Molecular Weight : ~48 kDa
Gene Aliases : G25K, CDC42Hs
Scientific Background : CDC42 is a member of the Rho family of GTPases and is part of cellular pathways fundamental to growth, differentiation and apoptosis (1). Downstream targets for CDC42 include those that regulate the actin cytoskeleton (e.g. WASP) and cellular stress pathways (e.g. PAK) as well as the coatomer protein complex and PAR6. CDC42 is involved in the G(1)-S progression of the cell cycle. CDC42 and its downstream effector mDia3 are involved in bi-orientation and stabilization of spindle microtubules attachment to kinetochores and regulate chromosome alignment and segregation during mitosis (2).
References :
1. Erickson, J.W. et al: Multiple roles for Cdc42 in cell regulation. Curr Opin Cell Biol. 2001 Apr;13(2):153-7.
2. Narumyia, S. et al: A new look at Rho GTPases in cell cycle: role in kinetochore-microtubule attachment. Cell Cycle. 2004 Jul;3(7):855-7.
Product Sheets
Research Areas: Cancer, Cell Cycle, Ser/Thr Kinases,