USP35, Active (U535-381G)
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Description : Recombinant human USP35 (395-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : Sf9 insect cells using baculovirus
Sequence : 395-end
Genbank Number : NM_020798
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 105 kDa
Gene Aliases : KIAA1372
Scientific Background : USP35 (ubiquitin carboxyl-terminal hydrolase 35) is a member of the peptidase C19 family of ubiquitin-specific proteases that plays a role in the regulation of mitosis. USP35 deubiquitinates Aurora B, which promotes stability and activates Aurora B. The reaction also antagonizes the APCCDH1-dependent K11-linked ubiquitination of Aurora B. USP35 expression is regulated by FoxM1 as well as Aurora B in the cell cycle (1). USP35 induced by miR let-7a can inhibit cell proliferation by inhibiting NF-κB activation via deubiquitination and stabilization of ABIN-2 protein (2). The miR let-7a-USP35-ABIN-2 pathway can be used as a target for novel cancer therapy.
References :
1. Park, J.Y. et al: USP35 regulates mitotic progression by modulating the stability of Aurora B. Nature Communications 9: 688, 2018.
2. Liu, C. et al: USP35 activated by miR let-7a inhibits cell proliferation and NF-κB activation through stabilization of ABIN-2. Oncotarget 6(29): 27891-906, 2015.
Product Sheets: (by Lot #):
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder