USP25, Protein (U525-31G)
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Description : Recombinant human USP25 (162-677 a.a.) was expressed in E. coli cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : E. coli cells
Sequence : 162-677 a.a.
Genbank Number : NM_013396
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : 85 kDa
Gene Aliases : USP21
Scientific Background : Ubiquitin carboxyl-terminal hydrolase 25 (USP25) belongs to the ubiquitin-specific proteases (USPs) family, and removes conjugated ubiquitin from substrates (1). USP25 processes, recycles ubiquitin to prevent the proteasomal degradation of proteins (1). It modifies tetraubiquitin chains via hydrolysing ‘Lys-63’ linked polyubiquitins to negatively regulate the inflammation mediated by IL-17 (2). A genome-wide association study of inflammatory bowel disease (IBD) identified the SNPs at USP25 sites (3).
References :
1. Valero R, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics. 1999 Dec 15;62(3):395-405.
2. Zhong B, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat Immunol. 2012 Nov; 13(11):1110-7.
3. Brant SR, et al. Genome-Wide Association Study Identifies African-Specific Susceptibility Loci in African Americans With Inflammatory Bowel Disease. Gastroenterology. 2017 Jan; 152 (1):206-217.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder