UCHL3, Active (U524-380H)
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Description : Recombinant full length human UCHL3, was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : Full length
Genbank Number : NM_006002
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 27 kDa
Gene Aliases : UCH-L3
Scientific Background : UCHL3 (Ubiquitin carboxyl-terminal hydrolase isozyme L3) is a thiol protease belonging to the deubiquitinating enzymes family and catalyzes the removal of ubiquitin from polypeptides. UCHL3 takes part in the regulation of apical membrane recycling by deubiquitinating ENAC in apical compartments (1). UCHL3 was found to hydrolyze mutant ubiquitin (UBB+1), a mutation that is associated with neurodegenerative diseases (2).
References :
1. Butterworth, M.B. et al: The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel. J Biol Chem. 282(52):37885-93, 2007.
2. Dennissen, F.J. et al: Mutant ubiquitin (UBB+1) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3). FEBS Letters 585:1873-3468, 2011.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder