USP7 (N-term), Active (U507-381H)
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Description : Recombinant human USP7 (25-580a.a.) was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : 25-580a.a.
Genbank Number : NM_003470
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 68 kDa
Gene Aliases : ISOT, Isopeptidase T
Scientific Background : USP7 (ubiquitin carboxyl-terminal hydrolase 7) belongs to the peptidase C19 family and deubiquitinates FOXO4, p53, MDM2, and WASH proteins, and regulates their activities through counteracting ubiquitin ligase activity of proteins such as HDM2 and TRIM27. USP7 was found to contribute to the stabilization and trans-activation of herpesvirus 1 transcriptional protein by strongly interacting with ICP0, a regulatory protein that stimulates lytic infection and the reactivation of viral genome (1). High expression of USP7 was correlated with the proliferation, migration and invasion capacities of hepatocellular carcinoma, indicating that the enzyme may play a role in promoting tumour progression (2).
References :
1. Boutell, C. et al: Reciprocal activities between herpes simplex virus type 1 regulatory protein ICP0, a ubiquitin E3 ligase, and ubiquitin-specific protease USP7. Journal of virology 79(19), 12342-54, 2005.
2. Wang, X. et al: Clinical Significance of Ubiquitin Specific Protease 7 (USP7) in Predicting Prognosis of Hepatocellular Carcinoma and its Functional Mechanisms. Medical science monitor:international medical journal of experimental and clinical research 24: 1742-1750, 2018
Product Sheets: (by Lot #):
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder