USP1, Active (U501-31G)
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Description : Recombinant human full-length USP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : Baculovirus in Sf9 insect cells
Sequence : Full length
Genbank Number : NM_003368
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 130 kDa
Gene Aliases : Ubiquitin carboxyl-terminal hydrolase 1
Scientific Background: Ubiquitin Specific Protease 1 (USP1) is a human deubiquitinase (DUB) that contains one of the largest catalytic domains within the USP family. USP1 plays a role in regulating cellular response to DNA damage and is also involved in the control of cell differentiation (1). USP1 regulates ubiquitination of Proliferating Cell Nuclear Antigen (PCNA) in response to DNA damage preventing unscheduled recruitment of error-prone TLS DNA polymerases [2]. USP1 expression is deregulated in certain types of human cancer and is thus emerging as a novel target in cancer therapy (3).
References :
1. Olazabal-Herrero, A. et al: Structure-function analysis of USP1: Insights into the role of Ser313 phosphorylation site and the effect of cancer-associated mutations on autocleavage. Mol Cancer. 14(1):33, 2015.
2. Huang, T.T. et al: Regulation of monoubiquitinated PCNA by DUB autocleavage. Nat Cell Biol. 8:339–47, 2006.
3. García-Santisteban, I. et al: USP1 deubiquitinase: Cellular functions, regulatory mechanisms and emerging potential as target in cancer therapy. Mol Cancer 12:91, 2013.
Product Sheets: (by Lot #):
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder