OTUB2, Active (O569-380H)
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Description : Recombinant human Ubiquitin thioesterase OTUB2 was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : Full Length
Genbank Number : NM_023112
Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 30 kDa
Gene Aliases : C14orf137, OTB2, OTU2
Formulation : 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Scientific Background : OTUB2 (Otubain 2) is a deubiquinating cysteine proteases that is encoded by the OTUB2 gene and belongs to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin-protein bond to cleave conjugated ubiquitin from proteins, which may play a role in the regulation of protein turnover by inhibiting degradation (1). OTUB-2 mediated deubiquitination of TRAF3 and TRAF6 may be involved in the regulation of virus-triggered type I interferon (IFN)2 induction pathways (2).
References :
1. Balakirev, M.Y. et al: Otubains: a new family of cysteine proteases in the ubiquitin pathway. EMBO Rep. 4, 5: 517–22, 2013.
2. Li, S. et al: Regulation of Virus-triggered Signaling by OTUB1- and OTUB2-mediated Deubiquitination of TRAF3 and TRAF6. The Journal of Biological Chemistry 285: 4291-4297, 2010.
Product Sheets: (by Lot #):
Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder