JosD2, Active (I560-380H)
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Description : Recombinant human JosD2 was expressed in E. coli cells using an N-terminal His tag.
Species : Human
Tag : His tag
Expression System : E.coli
Sequence : Full Length
Genbank Number : NM_138334
Specific Activity : Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Activity Assay, Western Blot
Molecular Weight : 20 kDa
Gene Aliases : SBBI54
Scientific Background : JosD2 (Josephin domain-containing 2) is a member of the MJD family of deubiquitinating enzymes (DUBs) that is localized to the cytoplasm and catalyzes the hydrolysis of the bond between the C-terminal glycine of the ubiquitin peptide and protein substrates. In vitro, JosD2 can cleave ubiquitin chains (1). Although JosD2 has been shown to have deubiquitinating activities, details of its substrate specificity are still mainly unknown (2).
References :
1. Seki, T. et al: JosD1, a membrane-targeted deubiquitinating enzyme, is activated by ubiquitination and regulates membrane dynamics, cell motility, and endocytosis. Journal of biological chemistry 288(24): 17145-55, 2013.
2. Tzvetkov, N. & Breuer, P: Josephin domain-containing proteins from a variety of species are active de-ubiquitination enzymes. Biol Chem 388(9):973-8, 2007.
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Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder