eRFP Protein (R620-30BH)
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Description :Recombinant full-length Bubble-tip anemone eRFP was expressed in E. coli cells using an N-terminal His tag.
Tag :His tag
Expression System:E.coli
Sequence :Full length
Genbank Number :AY130757
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight :~27 kDa
Gene Aliases :eqFP611
Scientific Background :eRFP (entacmaea quadricolor red fluorescent protein FP611) has the excitation maximum at 559 nm and the emission maximum at 611 nm (1). The fluorescence is constant within pH 4-10 (1). The eRFP proteins can be used for in vivo labeling in the eukaryotic systems through the expression in HeLa cells (2).
References :
1. http://www.uniprot.org/uniprot/Q8ISF8.
2. Wiedenmann, J., et al.: A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria). Proc. Natl. Acad. Sci. U.S.A. 99 (18), 11646-11651, 2002.
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Research Areas :AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cytoplasmic Tyrosine Kinases, ERK/MAPK Pathway, JAK/STAT Pathway, Lipid Kinases, Receptor Tyrosine Kinases